医学
渗透(HVAC)
阶段(地层学)
T细胞受体
免疫系统
肿瘤浸润淋巴细胞
腺癌
细胞瘤
断点群集区域
癌症
细胞
癌症研究
病理
受体
T细胞
内科学
免疫学
CD8型
古生物学
物理
热力学
生物
遗传学
作者
Chao Cheng,Thinh Tien Nguyen,Mabel Tang,Xinan Wang,Chongming Jiang,Yanhong Liu,Ivan P. Gorlov,Olga Y. Gorlova,John Iafrate,Michael Lanuti,David C. Christiani,Christopher I. Amos
标识
DOI:10.1016/j.jtho.2023.04.022
摘要
Introduction In recent years, the proportion of patients with NSCLC diagnosed at an early stage has increased continuously. Methods In this study, we analyzed samples and data collected from 119 samples from 67 early stage patients with NSCLC, including 52 pairs of tumor and adjacent non-neoplastic samples, and performed RNA-sequencing analysis with high sequencing depth. Results We found that immune-related genes were highly enriched among the differentially expressed genes and observed significantly higher inferred immune infiltration levels in adjacent non-neoplastic samples than in tumor samples. In survival analysis, the infiltration of certain immune cell types in tumor, but not adjacent non-neoplastic, samples were associated with overall patient survival, and excitingly, the differential infiltration between paired samples (tumor minus non-neoplastic) was more prognostic than expression in either non-neoplastic or tumor tissues. We also performed B cell receptor (BCR) and T cell receptor (TCR) repertoire analysis and observed more BCR/TCR clonotypes and increased BCR clonality in tumor than in non-neoplastic samples. Finally, we carefully quantified the fraction of the five histologic subtypes in our adenocarcinoma samples and found that higher histologic pattern complexity was associated with higher immune infiltration and low TCR clonality in the tumor-proximal regions. Conclusions Our results indicated significantly differential immune characteristics between tumor and adjacent non-neoplastic samples and suggested that the two regions provided complementary prognostic values in early-stage NSCLCs.
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