生物
胚胎干细胞
电池类型
转录组
基因表达
形态发生
计算生物学
细胞生物学
基因表达谱
胚胎心脏
细胞
基因
遗传学
基因表达调控
作者
Michaela Asp,Stefania Giacomello,Ludvig Larsson,Chenglin Wu,Daniel Fürth,Xiaoyan Qian,Eva Wärdell,Joaquín Custodio,Johan Reimegård,Fredrik Salmén,Cecilia Österholm,Patrik L. Ståhl,Erik Sundström,Elisabet Åkesson,Olaf Bergmann,Magda Bienko,Agneta Månsson‐Broberg,Mats Nilsson,Christer Sylvén,Joakim Lundeberg
出处
期刊:Cell
[Elsevier]
日期:2019-12-01
卷期号:179 (7): 1647-1660.e19
被引量:518
标识
DOI:10.1016/j.cell.2019.11.025
摘要
The process of cardiac morphogenesis in humans is incompletely understood. Its full characterization requires a deep exploration of the organ-wide orchestration of gene expression with a single-cell spatial resolution. Here, we present a molecular approach that reveals the comprehensive transcriptional landscape of cell types populating the embryonic heart at three developmental stages and that maps cell-type-specific gene expression to specific anatomical domains. Spatial transcriptomics identified unique gene profiles that correspond to distinct anatomical regions in each developmental stage. Human embryonic cardiac cell types identified by single-cell RNA sequencing confirmed and enriched the spatial annotation of embryonic cardiac gene expression. In situ sequencing was then used to refine these results and create a spatial subcellular map for the three developmental phases. Finally, we generated a publicly available web resource of the human developing heart to facilitate future studies on human cardiogenesis.
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