清脆的
功勋
核酸检测
计算机科学
水准点(测量)
计算生物学
检出限
核酸
纳米技术
材料科学
生物
化学
遗传学
色谱法
基因
计算机视觉
地理
大地测量学
作者
Reza Nouri,Ming Dong,Anthony J. Politza,Weihua Guan
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-03-03
卷期号:7 (3): 900-911
被引量:16
标识
DOI:10.1021/acssensors.2c00024
摘要
Clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid-sensing systems have grown rapidly in the past few years. Nevertheless, an objective approach to benchmark the performances of different CRISPR sensing systems is lacking due to the heterogeneous experimental setup. Here, we developed a quantitative CRISPR sensing figure of merit (FOM) to compare different CRISPR methods and explore performance improvement strategies. The CRISPR sensing FOM is defined as the product of the limit of detection (LOD) and the associated CRISPR reaction time (T). A smaller FOM means that the method can detect smaller target quantities faster. We found that there is a tradeoff between the LOD of the assay and the required reaction time. With the proposed CRISPR sensing FOM, we evaluated five strategies to improve the CRISPR-based sensing: preamplification, enzymes of higher catalytic efficiency, multiple crRNAs, digitalization, and sensitive readout systems. We benchmarked the FOM performances of 57 existing studies and found that the effectiveness of these strategies on improving the FOM is consistent with the model prediction. In particular, we found that digitalization is the most promising amplification-free method for achieving comparable FOM performances (∼1 fM·min) as those using preamplification. The findings here would have broad implications for further optimization of the CRISPR-based sensing.
科研通智能强力驱动
Strongly Powered by AbleSci AI