抗菌剂
共聚物
两亲性
化学
膜
胺气处理
共焦显微镜
抗菌活性
材料科学
细菌
生物物理学
聚合物
有机化学
生物化学
生物
细胞生物学
遗传学
作者
Min He,Yuming Zhou,Shuangxi Nie,Peng Lü,Huining Xiao,Yuan Tong,Qiang Liao,Ruili Wang
标识
DOI:10.1021/acs.jafc.8b01759
摘要
Two series of amphiphilic antimicrobial copolymers containing ciprofloxacin (CPF) and amine functional groups have been synthesized via free-radical copolymerization. The chemical structures of the different amine groups and the copolymer compositions have been systematically varied to study how the structure of the copolymer exerts an influence on the antibacterial activity. The viability of Escherichia coli in the presence of antimicrobial copolymers was observed by confocal laser-scanning microscopy (CLSM). CLSM as well as atomic-force microscopy (AFM) were applied to visualize changes in morphology of bacteria treated with antimicrobial copolymers and elucidate the antimicrobial mechanism of the antimicrobial copolymers. Morphological changes of bacteria observed via AFM and CLSM demonstrated that the antibacterial mechanism was due to the disruption of the bacterial membrane. The destruction of the cell membrane was also confirmed by the leakage of intracellular components, which had a strong absorbance at 260 nm. The inhibitory process was monitored by UV absorption dynamically.
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