Additional file 1: Figure S1. of Spatio-temporal profile, phenotypic diversity, and fate of recruited monocytes into the post-ischemic brain

CX3CR1GFP/+ and CCR2RFP/+ infiltrating monocytes after stroke in head-shielded CCR2RFP/+CX3CR1GFP/+ chimeric mice. (A) Coronal brain section images of BM CCR2RFP/+CX3CR1GFP/+ chimeric mice 3 and 14 days after MCAo showing anatomical localization of accumulated CCR2RFP/+ (red) and CX3CR1GFP/+ (green) cells in the ischemic hemisphere. (B) Cell number quantification of infiltrating CX3CR1GFP/+ and CCR2RFP/+ in the ischemic hemisphere 3 and 14 days after MCAo. (C) Representative images and orthogonal views of co-staining of CX3CR1GFP/+ Mo/MΦ (green) with the proliferation marker Ki67 (red) showing CX3CR1GFP/+ Mo/MΦ 14 days after MCAo. (D) CX3CR1GFP/+ cells (green) show three different phenotypes: cells with an amoeboid shape (left), perivascular cells with elongated shape located along the vessels (center panels; transverse and longitudinal vessel cross sections, respectively) and cells with ramified processes (right) 14 days after MCAo. Blood vessels were immunostained with the endothelial marker GLUT1 (red). (PDF 4803 kb)