The text cultivated in a pot to test through rape,study different soil nitrogen use firm nitrogen bacterial fertilizer tosoil microorganism amount groups of influence of quantity,nitrogen of carbon and fungus of microorganism under the lev- el.The result of study shows:In applying fertilizer the range,the amount of nitrogen constructed is different,the fertilizer ef- ficiency of the nitrogen-fixing bacteria is different too.In the medium-sized nitrogen level (N is 0.10 mg·kg~(-1)) when con- struct by result it is the best,exert a largest influence on carbon nitrogen of microorganism amount of soil one,fungus in- crease obviously too groups of quantity,but when the soil nitrogen level is too high or too low,firm nitrogen bacterial fertil- izer and microorganism fungus groups of influence of quantity obvious to soil microorganism amount carbon nitrogen.