Preparation of Quercetin-Al(III) Complex Molecularly Imprinted Polymer and Its Binding Characteristics

OBJECTIVE To prepare molecularly imprinting polymer(MIP) of quercetin-Al(Ⅲ) complex by using molecular imprinting technique(MIT),and study its binding characteristics.METHODS Using Al(Ⅲ)-quercetin complex as template molecule,α-methacrylic acid(MAA) as functional monomer and ethylene glycol dimethacrylate(EGDMA) as the crosslinker,a MIP with special molecule recognition properties was prepared.Its characteristics was studied by UV-Vis spectroscopy,IR spectroscopy,transmission electron microscopy and equilibrium binding experiments.RESULTS The interaction between quercetin and Al(Ⅲ) was investigated by UV-Vis spectroscopy,which indicated the interaction among quercetin,Al(Ⅲ) and α-methacrylic acid.The molecularyly imprinting for quercetin-Al(Ⅲ) showed good absorption selectivity and specificity to the complex of quercetin-Al(Ⅲ).CONCLUSION Complex molecularly imprinted polymer shows good binding characteristics to quercetin in the presence of Al(Ⅲ),and has good prospects in the application of separation and determination of quercetin.