Effect of recombinant human PTH 1-34 on the proliferation of osteoblasts and the gene expression of bone morphogenetic protein-7 and bone morphogenetic protein-9

Objective To study the effect of recombinant human parathyroid hormone(rhPTH1-34) on the proliferation of osteoblasts and the gene expression of bone morphogenetic protein-7(BMP-7) and bone morphogenetic protein-9(BMP-9).Methods Cultured osteoblasts were treated with different concentrations of rhPTH1-34(0,10-11,10-10,10-9,10-8,and 10-7mol/L),at the first 12h of a 24h interval.Cell proliferation was assessed using MTT colorimetric assay.The expression of BMP-7 and BMP-9 genes was examined with semi-quantitative RT-PCR.Results rhPTH1-34 promoted osteoblast proliferation(P0.01) and increased the expression of BMP-7 and BMP-9 genes with low dose and intermittent administration.Conclusion Low dose and intermittent administration of rhPTH1-34 induced osteoblast proliferation,probably in association with the expression of BMP-7 and BMP-9.