Biotransformation RSA to hydrocortisone:improvement of adding substrate

The selected mode of Tween-80/glycerol/RSA/PBS(v(Tween-80)∶v(glycerol)∶w(RSA)∶v(PBS)=1∶3∶1∶25)for addition of the substrate RSA was developed.The mode was used in the procedure of the bioconversion from RSA to HC by cooperative mycelia Abisidia coerulea and Curvularia lunata,making the substrate concentration accumulated in the media be up to 5 g/L with the considerable yield of HC,compared with the 2 g/L in the traditional one with the type of RSA/ethanol(w(RSA)∶v(ethanol)=1∶25).The concentration of the substrate RSA was 3 g/L,the system of the cooperative mycelia media could be repeatedly used 3 times with the HC produced yield of 70% in a preparative-scale experiment at the gram level,the recovery ratio of product HC from RSA was up to 52%.The novel mode of addition substrate in the bioconversion process showed a reproducible and stable process.