Studies of Cellular Immune and its Influence on Bone Marrow Hemapoietic Function in Aplastic Anemia Patients

Objective: To explore the cellular immune condition of aplastic anemia (AA) patients and its influence on pathogenesis of AA. Methods: Phenotype of T lymphocytes was assayed with indirect immunofluorescence assay and serum TNF αlevel was determined with RIA in 29 cases. The bone marrow (BM) CFU GM colony growth and the influence of AA BM cells and serum on normal BM CFU GM were observed by methylcellulose micromethod. According to this, AA patients were classified. Results: Phenotype of T lymphocytes and serum TNF αlevel in immuno mediated AA were obviously different to normal controls. The percentage of HLA DR+cells In stem cell defective AA were more than that in normal controls (P0.05), while the ratio of CD4/CD3 was lower (P0.01). Conclusions: There were cellular immune abnormality in most of the immuno mediated AA, in some of the stem cell defective AA and in a few of microenvironmental defective AA.