The denitrification of UNITANK was studied by using activated sludge mathematical model ASM2D,through which the basic factors affecting denitrification can be understand,the denitrification ability can be predicted and the denitrification in sedimentation area can be early alarmed as well.The results showed the triple-tank structure was not good for nitrogen removal of denitrification;tank B with anoxic condition can realize partially denitrification;Denitrification carried out mainly in sedimentation area,but as carbon source was deficient,denitrification efficiency was not high and further denitrification could lead to the risk of sludge floating.