The Approach on the Main Influencing Factors of Chromogenic Substrate System of the Indirect ELISA

Results of the different enzyme linked immunosorbent assay (ELISA) can be easily affected by many factors which include chromogenic substrate system. This paper discussed the influences on chromogenic status by ingredients, contents and reaction conditions of the commonly used TMB substrate reagent solution system, and finally made a general adjustment to the parameters of the chromogenic substrate system, including the pH, substrate ingredients, contents and reaction time. And by using this way the research established a foundation to define a chromogenic substrate system for each different ELISA and to set up a stable ELISA system.