Study on the Quality Controlling Method of Chuanxiong Medicine Material by HPLC

Objective: To establish an HPLC method for evaluating and controlling the quality of Chuanxiong medicine material. Method: The samples were pretreatment by solvent extract, and HPLC separation was carried out on an Agilent Extend - C18 column by a linear gradient elution with ratios of methanol - 1% acetic acid solution changed from 35: 65 to 80: 20 within 40 min. The flow rate of the mobile phase is 0. 8 mL ·?min-1. The detection wavelength is 290 nm. Result: The main effective components in the sample were separated in 35 min with good resolution. Conclusion: The HPLC method developed here is stable and reliable.