Determination of Gossypol of Different Types of Gland in Cotton by High-Performance Liquid Chromatography

The comparison studies on gossypol of different genetic types of pigment gland in the seeds of cotton newly bred, including the special and common glandless and glanded ones, was conducted through highperformance liquid chromatography (RPHPLC) on a C18 Column with methanol 0.5% acetic acid aqueous solution, 90∶10 as mobile phase at a flow rate of 0.8mL·min-1 and UV detection at 254 nm. This method was shown to be highly reproducible, with precision (as RSD) and accuracy (as RME) 10%, both intraday and interday. Absolute recoveries 100.4%～103.2%. The results revealed remarkable differences among the seeds of the different gland genetic varieties or species of cotton, The studies displayed the precious resources of different gland of extraordinary cotton.