毛囊
生物
表皮(动物学)
转录组
细胞生物学
干细胞
细胞分化
形态发生
发育生物学
再生(生物学)
角质形成细胞
细胞
基因表达
基因
遗传学
解剖
细胞培养
作者
Simon Joost,Amit Zeisel,Tina Jacob,Xiaoyan Sun,Gioele La Manno,Peter Lönnerberg,Sten Linnarsson,Maria Kasper
出处
期刊:Cell systems
[Elsevier]
日期:2016-09-01
卷期号:3 (3): 221-237.e9
被引量:325
标识
DOI:10.1016/j.cels.2016.08.010
摘要
The murine epidermis with its hair follicles represents an invaluable model system for tissue regeneration and stem cell research. Here we used single-cell RNA-sequencing to reveal how cellular heterogeneity of murine telogen epidermis is tuned at the transcriptional level. Unbiased clustering of 1,422 single-cell transcriptomes revealed 25 distinct populations of interfollicular and follicular epidermal cells. Our data allowed the reconstruction of gene expression programs during epidermal differentiation and along the proximal-distal axis of the hair follicle at unprecedented resolution. Moreover, transcriptional heterogeneity of the epidermis can essentially be explained along these two axes, and we show that heterogeneity in stem cell compartments generally reflects this model: stem cell populations are segregated by spatial signatures but share a common basal-epidermal gene module. This study provides an unbiased and systematic view of transcriptional organization of adult epidermis and highlights how cellular heterogeneity can be orchestrated in vivo to assure tissue homeostasis.
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