Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA‐MB‐231 In Vitro

Objective. To evaluate the effect of petroleum ether extracts of Curcuma zedoaria on the proliferation of human triple negative breast cancer cell line MDA‐MB‐231. Methods. The reagents were isolated from Curcuma zedoaria by petroleum ether fraction. It was assayed by CCK8 for MDA‐MB‐231 cellular viability with various concentrations and days, cell cycle analyses, Western Blot analysis, and Realtime Reverse Transcriptase PCR analyses for chemokines molecules including E‐cadherin, and E‐selectin, and adhesion molecules including CCR7, SLC, SDF‐1, and CXCR4. Epirubicin was used as control in the study. Results. MDA‐MB‐231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria ( P < 0.05), and the inhibition rate was dependent on concentrations and time. Petroleum ether extracts of Curcuma zedoaria as well as Epirubicin produce a significant G0/G1 cell cycle arrest. The level of expression of proteins E‐cadherin and E‐cadherin mRNA was significantly increased, while proteins SDF‐1, CCR7, and CXCR4 mRNA were decreased after being incubated with petroleum ether extracts of Curcuma zedoaria at the concentrations of 300 μ g/mL than control ( P < 0.05). The differences were that the protein CXCR4 mRNA expression level was higher than vehicle. Conclusions. MDA‐MB‐231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria .