Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes

In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.