生物
菌丝体
原基
真菌
植物
担孢子
担子果
擔子菌門
寄主(生物学)
发芽
孵化
生态学
生物化学
分类学(生物学)
基因
作者
Jaturong Kumla,Boonsom Bussaban,Nakarin Suwannarach,Saisamorn Lumyong,E. Danell
出处
期刊:Mycologia
[Taylor & Francis]
日期:2012-05-01
卷期号:104 (3): 597-603
被引量:17
摘要
Phlebopus portentosus is a popular wild edible ectomycorrhizal fungus in northern Thailand. In general ectomycorrhizal fungi produce basidiomes when associated with a host plant. In this paper mycelium growth and basidiome production of P. portentosus were examined in pure culture both in vitro and in pot-culture experiments. Five mycelial strains of P. portentosus were isolated from basidiomes and used in the experiments. The mycelia grew fastest on sorghum grains supplemented with fungal-host solution. The mycelia produced sclerotia-like structures after 3 wk incubation in darkness at 30 C. All strains of P. portentosus had the ability to form primordia. The primordia were formed under lowered temperature, high humidity and a 12 h photo-period. They developed to mature basidiomes after 8–12 d in in vitro. In the pot-culture primordia were found after 28–35 d incubation in the greenhouse and mature basidiomes released basidiospores within 6–8 d. Basidiospores were germinated on fungal-host medium and formed mycelial colonies. This fungus showed an ability to produce basidiomes even 2 y after the original isolation from tissues. This research provides valuable information concerning the techniques and protocols for the large scale commercial production of P. portentosus basidiomes in the absence of a host plant.
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