Study on T-Cell and T-Helper Cell Subpopulation Function in Children with Recurrent Tonsillitis in Remission Period

Objective To study the state of T-cell and T-helper cell subpopulation in children with recurrent tonsillitis(RT) in remission period and make clear its role on pathogenesis in children with RT.Methods The expression of membrane molecule on T-cell subsets was analyzed in 27 RT children and 21 control health children by using immunofluorescence and flowcytometry,the levels of interferon-γ(IFN-γ) and interleukin-4(IL-4) in culture supernatant of peripheral blood mononuclear cells(PBMC) were detected by enzyme linked immunoadsorbent assay(ELISA).Results The CD4~+ and CD3~+ cell were significantly lower in RT children than those of the controls(P0.01),but no difference in CD8~+ cell between the patients and controls(P0.05),a ratio of CD4~+/CD8~+ was disproportion(P0.01);the level of IFN-γ relating to function of Th1-cell in RT children was significantly lower than that of the controls(P0.01),but no difference in IL-4 relating to function of Th2-cell between the patients and controls(P0.05),the ratio of Th1/Th2 was disproportion(P0.01).Conclusion There are dysfunction of T cell in RT children in remission period;The abnormal Th1 cell function may play an important role on pathogenesis.