Immunohistochemical Study of Dendritic Cells in Human Breast Cancer

Objectives To observe the immunohistochemical changes of dendritic cells(DCs) in human breast cancer.Methods The distribution and morphological changes of DCs in 20 cases of human breast cancer were observed with immunohistochemitry,using CD 1C+ monoclonal antibody as specific marker for DCs,and the expression of p53 was determined also.Results The square density (SD, 4.55±1.32) and mean optic density (MOD, 0.13±0.03) of CD 1C+ positive product in DCs of human breast cancer were lower than those (11.16±5.32, 0.27± 0.07) in normal breast tissue respectively (P0.05),and the number of DCs declined greatly in breast cancer tissue compared with normal breast tissue. The positive expression rate of p53 protein was 60% in 20 cases of breast cancer thssue,but negative in normal breast tissue.Conclusion DCs and p53 protine may play an important regulatory role in initiating immune response against breast cancer.