Effects of combined radiation-burn injury on bone marrow stromal cells in mice

Objective To investigate the effects of combined radiation burn injury on bone marrow stromal cells in mice. Methods Mice were treated with 6.0 GY  ray radiation and 15% (TBSA) Ⅲ° burn. The culture of CFU GM and bone marrow stromal adherent cells were performed. Cell cycle, DNA content and the expression of adhesion molecules were detected by flow cytometry. Results ①The adherent capacity of bone marrow stromal cells and the numbers of CFU F after combined radiation burn injury were significant decreased. ②Three days later, cultured stromal adherent cell inhibited the growth of CFU GM, but when the agar layer existed that inhibition were weaken or disappear. ③The cell proportion of G 0 and G 1 was decreased, and the proportion of S and G 2+M was increased after combined injury. ④The DNA content in the bone marrow stromal cells was significantly lower in the combined injury group after 3 days. ⑤The expression of cell adhesion molecules including vascular cell adhesion molecule 1 (VCAM 1), fibronection (Fn), laminin (Ln), and collagen type Ⅳ (Col Ⅳ) on bone marrow stromal cell were decreased in combined radiation burn injury. Conclusions The damage of bone marrow stromal cells in bone marrow hematopoietic inductive microenvironment might be one of the most important factors in hematopoietic disorder in combined radiation burn injury.