Determination of loganin in liuwei dihuang oral liquid by HPLC

Aim To determine loganin in liuwei dihuang oral liquid by HPLC.Methods Octadecylsilane chemically bonded silica was used as filling material.The mobile phase is tetra hydrofuran-methyl lyanide-carbinol-0.05% phosphoric acid solution(1∶8∶4∶87);The detection wavelength is at 236 nm.The temperature of detection wavelength column is 40℃.The theoretical plates is not lower than 4000 as calculated according to loganin area.The keeping time of loganin is 17~18 minutes.The quantity of inputting samples is 10 μl.Results The linear range is from 5~26 mg·L~(-1) for loganin,Y=20878.05X-224.51,r=0.9999.The average recovery of loganin for the added samples is at 95%~100%.Conclusion This experiment shows that this method is convenient,quick,exact,sensitive and reproductive.