Study on Extraction and Determination of Hesperidin from Different Peels of Citrus and its Hydroxyl Radical Scavenging Effects

Objective To study the determination method of hesperidin content by spectrophotometer and extraction technology of hesperidin from peels of citrus.Methods Analysis technology: The type of solvent,temperature,quantity of indicator and the time of reaction were investigated through orthogonal tests of L9(34);Extraction technology: The concentration of ethanol,quantity of ethanol,time and temperature of extraction were investigated through orthogonal tests of L9(34).In addition,the scavenging effect of flavanone from peels of citrus on hydroxyl radical was studied.Results The optimum technical conditions for determining the content of hesperidin by spectrophotometer were that: hesperidin was immersed with 5% Al(NO3)3(m:V)at 20℃ for 20 min and the maximum absorbance wavelength was 400 nm;the optimum technical conditions of extracting hesperidin were that: 70% ethanol was used as solvent,and the hesperidin was extracted at 50℃ for 3 h when the powers was stirred at medium-speed,the mass ratio of peels weight to 70% ethanol solution was 1:20(m:V).In addition,total flavanone from peels of citrus had effects on scavenging of hydroxyl radicals.Conclusion The content of hesperidin in peels is the highest by improved extraction technology.