体内
淋巴细胞
生物
分子生物学
体外
T淋巴细胞
MTT法
抗体
胸腺细胞
免疫学
T细胞
免疫系统
生物化学
生物技术
作者
Yanping Wang,Xiaoyu Li,Chun-Qing Song,Zhi-Bi Hu
出处
期刊:PubMed
日期:2002-03-01
卷期号:23 (3): 263-6
被引量:39
摘要
To investigate the effect of astragaloside IV (ASI) on T, B lymphocyte proliferation, antibody production, and cytokines produced by murine peritoneal macrophages.MTT assay was used to determine T, B lymphocyte proliferation and quantitative hemolysin spectrophotometry (QHS) assay was applied to test antibody production; IL-1 production was measured by thymocyte proliferation assay; TNF-alpha production was determined by the cytotoxicity assay against L929 cells.1) In vivo, ASI 50-200 mg/kg ig for 7 d increased T lymphocyte proliferation and antibody production, and ASI 50-100 mg/kg ig for 7 d increased B lymphocyte proliferation but ASI 200 mg/kg had no effect on B lymphocyte proliferation; 2) In vitro, ASI increased T, B lymphocyte proliferation only at 100 nmol/L; 3) ASI increased IL-1 activity at 1 nmol/L in vitro, but decreased it at 100 and 1000 nmol/L; 4) ASI inhibited TNF-alpha activity with or without LPS-stimulation in vitro.ASI increased T, B lymphocyte proliferation and antibody production in vivo and in vitro; but inhibited productions of IL-1 and TNF-alpha from peritoneal macrophages in vitro.
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