Determination of Ginsenosides in Cell-wall-broken Powder of Red Ginseng by HPLC

Objective To develop a method for the determination of ginsenosides in cell-wall-broken powder of red ginseng.Methods High performance liquid chromatography(HPLC)was used for the determination of ginsenosides such as ginsenoside Rg1,ginsenoside Re,and ginsenoside Rb1.Results The results of ginsenosides determination in 13 batches of cell-wall-broken powder of red ginseng showed that ginsenoside Rg1 had a good linearity in the range of 1.012～10.120 μg with average recovery being 101.4% and RSD being 1.7%,ginsenoside Re had a good linearity in the range of 0.653 4～6.534 μg with average recovery being 95.0% and RSD being 1.8%,and ginsenoside Rb1 had a good linearity in the range of 1.019～10.190 μg with average recovery being 104.9% and RSD being 0.8%.Conclusion The established method is simple and rapid,which can be used for the quality control of cell-wall-broken powder of red ginseng.