Determination of the Content and the Related Compounds in Cuscohygrinolis α-Acetylbenzoacetate by HPLC-DAD-ELSD

OBJECTIVE To establish a HPLC-DAD-ELSD method for the deter mination of the content of cuscohygrinol,acetylmandelic acid and other related compounds in cuscohygrinolis α-acetylbenzoacetate.METHODS The HPLC method was conducted on a Waters symmetry C18 column(4.6 mm×150 mm,5 μm)and coupled with ELSD and DAD detector at 219 nm.The mobile phase consisted of methanol-0.25% of pentafluoropropionic acid(contains 30 mmol.L-1 of ammonium formate),and the column temperature was at room temperature.RESULTS A good resolution was obtained among cuscohygrinol,acetylmandelic acid,cuscohygrinolis α-acetylbenzoacetate and other related compound.The linear ranges of cuscohygrinol,acetylmandelic acid and cuscohygrinolis α-acetylbenzoacetate were 0.016 32-0.163 2,0.005 65-0.113 and 0.228 2-2.282 g.L-1,respectively.The average recovery of cuscohygrinol,acetylmandelic acid and cuscohygrinolis α-acetylbenzoacetate were 100.1%,101.2% and 99.6% respectively.CONCLUSION A simple,accurate and sensitive HPLC-DAD-ELSD method to determine the content of cuscohygrinol,acetylmandelic acid and other related compounds in cuscohygrinolis α-acetylbenzoacetate was established.