神经科学
生物
电生理学
细胞生物学
肠神经系统
祖细胞
祖细胞
神经干细胞
原代培养
肌间神经丛
干细胞
细胞培养
免疫组织化学
免疫学
遗传学
作者
Yonggang Zhang,Wenhui Hu
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 63-71
被引量:3
标识
DOI:10.1007/978-1-0716-1437-2_6
摘要
In the enteric nervous system, there exist a huge number of local intrinsic neurons, which control the gastrointestinal functions. Culture of enteric neurons provides a good model system for physiological, electrophysiological, and pharmacological studies. Here, we describe two methods to obtain sufficient enteric neurons from mouse myenteric plexuses by directly culturing primary neurons or inducing neuronal differentiation of enteric neural stem/progenitor cells.
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