Design and synthesis of a deep tissue penetrating near-infrared two-photon fluorescence probe for the specific detection of NQO1

NAD(P)H:quinone oxidoreductase 1 (NQO1) is overexpressed in a broad range of human tumors but remains difficult to study. Herein, we report a novel two-photon fluorescent probe with NIR emission for NQO1 detection. The probe demonstrated superior analytical performance with a large Stokes shift and deep tissue penetration.