平衡
铁稳态
铁质
双氢青蒿素
癫痫
双光子激发显微术
化学
体内
细胞生物学
生物
药理学
神经科学
医学
荧光
生物化学
病理
疟疾
新陈代谢
有机化学
生物技术
青蒿素
物理
恶性疟原虫
量子力学
作者
Chenwen Shao,Yani Liu,Zhang-Peng Chen,Yajuan Qin,Xueao Wang,Xueting Wang,Chao Yan,Hai‐Liang Zhu,Jing Zhao,Yanlong Qian
标识
DOI:10.1016/j.chembiol.2021.12.006
摘要
Imbalanced iron homeostasis plays a crucial role in neurological diseases, yet direct imaging evidence revealing the distribution of active ferrous iron (Fe2+) in the living brain remains scarce. Here, we present a near-infrared excited two-photon fluorescent probe (FeP) for imaging changes of Fe2+ flux in the living epileptic mouse brain. In vivo 3D two-photon brain imaging with FeP directly revealed abnormal elevation of Fe2+ in the epileptic mouse brain. Moreover, we found that dihydroartemisinin (DHA), a lead compound discovered through probe-based high-throughput screening, plays a critical role in modulating iron homeostasis. In addition, we revealed that DHA might exert its antiepileptic effects by modulating iron homeostasis in the brain and finally inhibiting ferroptosis. This work provides a reliable chemical tool for assessing the status of ferrous iron in the living epileptic mouse brain and may aid the rapid discovery of antiepileptic drug candidates.
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