血管生成
蛋白激酶B
焦点粘着
脐静脉
细胞生物学
细胞生长
细胞外基质
伤口愈合
人脐静脉内皮细胞
医学
细胞迁移
内皮干细胞
信号转导
癌症研究
生物
细胞
免疫学
生物化学
体外
作者
Michika Fukui,Yuki Matsuoka,Shigeru Taketani,Koichiro Higasa,Masakatsu Hihara,Atsuyuki Kuro,Natsuko Kakudo
出处
期刊:Annals of Plastic Surgery
[Ovid Technologies (Wolters Kluwer)]
日期:2022-10-17
卷期号:89 (6): e51-e59
被引量:1
标识
DOI:10.1097/sap.0000000000003298
摘要
Negative pressure has been used as a preferred therapy for wound healing; however, the mechanisms by which negative pressure promotes tissue restoration remain unclear. In the present study, RNA-sequencing analysis was performed to identify differentially expressed genes in human umbilical vein endothelial cells (HUVECs) exposed to negative pressure. Cell viability and DNA synthesis were examined using the cell counting kit-8 assay and bromodeoxyuridine incorporation, respectively. Cell migration was assessed using tube formation, Transwell, and wound healing assays. Activity of the serine/threonine kinase (AKT) signaling pathway was also examined by measuring the levels of phospho-paxicillin, phospho-focal adhesion kinase (p-FAK), and p-AKT1. The exposure of HUVECs to negative pressure enhanced cell proliferation and DNA synthesis. Negative pressure enhanced the migration and invasion of HUVECs, which was accompanied by upregulation of genes involved in angiogenesis, extracellular matrix organization, and cytoskeletal organization. The mRNA levels of growth factors, including placental growth factor and platelet-derived growth factor B, also increased. In addition, phosphorylation of paxicillin, focal adhesion kinase, and AKT increased under negative pressure. Collectively, the findings of this study demonstrated that negative pressure stimulates the angiogenic activity of HUVECs by increasing their proliferation and migration via activation of the AKT signaling pathway.
科研通智能强力驱动
Strongly Powered by AbleSci AI