Synthesis and Characterization of a Nanoclay Reinforced Gelatin‐Based Hybrid Hydrogel

ABSTRACT Bentonite clay nanoparticles assume a pivotal role in 3D bioprinting and tissue engineering by augmenting the mechanical rigidity and biological efficacy of hydrogels. In this investigation, Span80 was employed as a surfactant to facilitate the synthesis of uniformly sized bentonite nanoparticles measuring approximately 700 nm in diameter. The resultant hybrid hydrogel displaced a marked increase in compressive modulus, achieving a peak value of 17.5 kPa, including 1% bentonite twice that of the unmodified gelatin methacryloyl (GelMA). The discernible enhancements in the physical and biological characteristics of the hydrogel underscore its considerable potential for applications in tissue engineering. This includes heightened mechanical rigidity, robust cell viability, and a meticulously regulated degradation rate. While further examinations are imperative to evaluate the viability of the developed hydrogel comprehensively, its auspicious physical and biological attributes strongly suggest its potential utility in the domain of tissue engineering and bioprinting.