成纤维细胞
医学
CD90型
免疫组织化学
病理
免疫荧光
逆转录聚合酶链式反应
前庭系统
生物
信使核糖核酸
细胞培养
免疫学
细胞生物学
干细胞
川地34
抗体
遗传学
放射科
基因
作者
Paramita Baruah,Jennifer L. Marshall,Philip R. Jones,Triin Major,Valentina Pucino,John D. O’Neil,Meriam Nefla,Helen M. McGettrick,Peter Monksfield,Richard Irving,Christopher D. Buckley
出处
期刊:Otology & Neurotology
[Ovid Technologies (Wolters Kluwer)]
日期:2023-09-15
卷期号:44 (10): e755-e765
标识
DOI:10.1097/mao.0000000000004011
摘要
Background and aim Vestibular schwannomas (VSs), despite being histologically benign, cause significant morbidity because of their challenging intracranial location and the propensity for growth. The role of the stroma and particularly fibroblasts, in the progression of VS, is not completely understood. This study examines the profile of fibroblasts in VS. Methods Seventeen patients undergoing surgical excision of VS were recruited into the study. Reverse transcription with quantitative polymerase chain reaction (RT-qPCR) was performed on VS tissue samples and fibroblast-associated molecules examined. Immunofluorescence and immunohistochemistry in VS tissue were used to study the expression of fibroblast markers CD90 and podoplanin in situ. Fibroblast cultures were established from VS, and RT-qPCR analysis was performed on a panel of fibroblast markers on VS and control tissue fibroblasts. Results Several fibroblast-associated molecules including members of galectin family and matrix metalloproteinases were found to be expressed in VS tissue on RT-qPCR analysis. In situ, expression of CD90 and podoplanin was observed in VS tissue both on immunohistochemistry and immunofluorescence. RT-qPCR analysis of fibroblasts from VS and control vestibular neuroepithelium (NE) showed a higher expression of several molecules of the galectin and matrix metalloproteinases family on VS fibroblasts compared with NE fibroblasts. Conclusion This work examines fibroblasts from VS and shows qualitative differences from NE fibroblasts on RT-qPCR. Further understanding of the fibroblast function in the progression of VS will potentially unveil new targets to manage VS growth.
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