碱性磷酸酶
肝移植
染色
男科
无人机
移植
组织学
末端脱氧核苷酸转移酶
丙氨酸转氨酶
高架桥
生物
内科学
标记法
免疫组织化学
医学
化学
病理
酶
生物化学
遗传学
作者
Takahiro Enjoji,Akihiko Soyama,Manabu Fukumoto,Peilin Li,Kunihito Matsuguma,Hajime Imamura,Yasuhiro Maruya,Takanobu Hara,Hajime Matsushima,Tota Kugiyama,Tomohiko Adachi,Masaaki Hidaka,Susan Hamamoto,Shiro Takashima,Takahiro Maeda,Kengo Kanetaka,Masaaki Hidaka
标识
DOI:10.1016/j.transproceed.2023.08.018
摘要
Recently, the successful delivery of organs for transplantation using drones was reported. We investigated the influence of transportation by drones on the quality of liver grafts using a rat model.Livers of 12 rats (8 and 32 weeks old) were divided into 2 groups of six. Livers were split into 2 parts and allocated to the drone or control groups (both n = 12). The drone experiment was conducted between islands in Nagasaki Prefecture, Japan. The distance between the islands was 12 km. Livers of the drone group were transported by a multicopter at a speed of 30 km-40 km/h over 60 m above sea level. Transported liver quality was analyzed by histology, and biochemistry data were compared between groups.Cold ischemia time did not differ between groups (902 min and 909 min, respectively). There were no differences in macroscopic findings regarding coloration and damage between groups. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in preservation fluid were graft weight-corrected and compared, and no significant differences were found between groups: AST/g (4.61 vs 4.81 IU/L), ALT/g (2.78 vs 2.92 IU/L), and ALP/g (39.1 vs 37.0 IU/L). Immunochemical staining showed no significant difference between groups for terminal deoxynucleotidyl transferase dUTP nick and labeling staining (141 vs 113 cells), CD163 (818 vs 870 cells), and TNF-α (1.25 vs 1.41 scores).The simulation experiment of organ transport for transplantation by drones was successfully conducted. There were no differences in the quality of livers transported by drones or other means. Further studies including large-animal experiments could lead to future clinical applications.
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