Identification and analysis of microplastics in human lower limb joints

Microplastics (MPs) have been detected in various human tissues, including the liver, placenta, and blood. However, studies about MPs in the human locomotor system are limited. This study evaluated the presence of MPs in the synovium of 45 patients undergoing hip or knee arthroplasty using micro-Fourier transform infrared spectroscopy, scanning electron microscopy, and Raman microscopy and investigated their association with clinical indicators and local cellular responses. A total of 343 MPs of nine common types were identified, with a mean abundance of 5.24 ± 2.07 particles/g and ranging from 1.16 to 10.77 particles/g. Although there was no clear correlation between MP abundance and demographics, MP abundance was higher in hip samples than in knee samples. In addition, a potential association was observed between MP abundance and specific clinical diagnoses. Transcriptomic analysis revealed that a three-fold increase in MP abundance corresponded to enhanced local cellular stress responses, particularly heat shock protein reactions. Our findings demonstrate the presence of MPs in human joints and suggest that further studies are needed to explore the intricate associations between MPs and anatomical location, clinical diagnosis, and local cellular responses.