[Electroacupuncture of specific acupoints of Shaoyang Meridian relieves progression of chronic migrain by down-regulating expression of P2Y12 receptor in rats].

OBJECTIVES: To observe the effect of electroacupuncture (EA) at specific points of the Shaoyang Meridian on mechanical pain threshold, and expressions of P2Y12 receptor (P2RY12), ionic calcium binding molecule 1 (Iba-1), calcitonin gene-related peptide (CGRP) and c-fos in rats with chronic migraine (CM) , so as to explore its mechanisms underlying prevention and treatment of CM. METHODS: day of modeling, 2 h before the intervention, the mechanical pain thresholds of the periorbital region of the eye and the plantar region of the hindlimb in each group were measured using a von Frey filament. The number of P2RY12 positive cells and the expression of P2RY12 mRNA in the caudal nucleus of the trigeminal spinal tract (TNC) were detected using immunofluorescence staining and real-time fluorescence quantitative PCR, separately, and the expression levels of P2RY12, Iba-1, CGRP, and c-fos proteins in the TNC tissue were detected using Western blot. RESULTS: <0.01). CONCLUSIONS: EA of the specific points of Shaoyang Meridian can prevent the progression of migraine, and reduce pain, which may be related to its function in down-regulating the expressions of P2RY12 protein and mRNA, Iba-1, and CGRP and c-fos proteins (central sensitization-related indexes) in TNC.