脂肪生成
脂解
脂滴包被蛋白
安普克
3T3-L1
脂肪生成
五味子
化学
脂肪甘油三酯脂肪酶
脂肪细胞
内分泌学
内科学
脂滴
磷酸化
生物化学
脂肪组织
蛋白激酶A
生物
医学
替代医学
中医药
病理
作者
Miran Jang,Soo‐Im Choi
摘要
In this study, lignans of Schisandra chinensis fruits (SCF) were profiled using HPLC–MS/MS, and the inhibitory effects of nine of these lignans were evaluated on triglyceride (TG) accumulation. We then examined the effects and molecular mechanisms on adipogenesis and lipolysis of schisandrin C (SC), which most inhibited TG levels during adipogenesis of 3T3-L1 cells. Treatment of 3T3-L1 cells with SC markedly decreased adipocyte differentiation but did not influence cell proliferation. During adipogenesis, SC significantly reduced total lipid and TG contents and down-regulated the mRNA expressions of C/EBPα, PPARγ, SREBP1c, aP2, and FAS. In addition, SC significantly increased p-AMPK, and this activation regulated the protein levels of major adipogenic transcription factors (PPARγ and C/EBPα). Furthermore, SC lowered the mRNA expressions of HSL and perilipin and inhibited pancreatic lipase levels, which are both related to lipolysis. Practical applications Our results indicate that SC regulates lipogenesis and lipolysis by increasing AMPK phosphorylation and suggest that it may be beneficial for preventing obesity and related metabolic diseases. Thus, this study proposes a mechanical basis for developing SC-containing foods as a beneficial dietary strategy.
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