Visualization of biological nano-objects with the help of multiphoton microscopy

The results of studies of exosome visualization obtained using multiphoton fluorescence spectroscopy for healthy volunteers and patients with colorectal cancer are presented. Standard centrifuging technology was used to isolate exosomes from blood plasma. Exosome samples were investigated using the femtosecond laser pumping at the wavelength of 760 nm with and without FITC (Fluorescein isothiocyanate) labels. In particular, the exosomes with CD9 surface glycoprotein of the tetraspanin family was shown to be studied without labeling using FLIM (fluorescent lifetime microscopy).