A Reactive Oxygen Species (ROS) Activated Hydrogen Sulfide (H2S) Donor with Self-Reporting Fluorescence

Hydrogen sulfide (H₂S) is an important cellular signaling molecule, and its physiological and pathophysiological properties have been under intensive investigation. In this study, a novel ratiometric fluorescent H₂S donor (HSD-B) has been developed, which exhibited the following advantages: (i) scavenging ROS and producing H₂S simultaneously; (ii) providing ratiometric fluorescence for visualization and quantification of H₂S releasing; and (iii) targeting mitochondrion specifically. Moreover, it demonstrated protective effects on myocardial ischemia reperfusion injury in a cellular model. These attractive features promise this HSD-B as a fluorescent H₂S donor for future research studies.