Difference between capacitation and frozen-thawed cell actin expression in boar sperm

[Objective] The study was done in order to analyze the difference between capacitation and frozen-thawed boar sperm cells actin expression.[Method] After SDS-PAGE separation of the capacitation and frozen-thawed boar sperm,western blotting analysis was carried out to detect the distinction between capacitation and frozen-thawed boar sperm cell actin.[Result] Monomer molecular weight of fresh and frozen-thawed boar sperm cells actin was about 43 ku,but capacitation boar sperm cells actin was about 43 ku and 86 ku.[Conclusion] Monomer molecular weight of frozen-thawed boar sperm cells actin was still 43 ku,but there was a decrease in number;a new actin subunit appeared in capacitation boar sperm,and the molecular weight was 86 ku.