Inhibitory Effects ofAngelica pubescensf.biserrataon 5-Lipoxygenase and Cyclooxygenase

Linoleic acid, osthol, osthenol and two polyacetylenes, falcarindiol and 11(S),16(R)-dihydroxyoctadeca-9Z,17-diene-12,14-diyn-1-yl acetate were found to be the most active compounds responsible for the inhibitory activity of the di-chloromethane extract of the roots of Angelica pubescens f. biserrata on 5-lipoxygenase (5-LO) and cyclooxygenase (COX-1) in vitro. They showed prominent inhibitory effect on 5-LO with IC50 values of 27.9 µM, 36.2 µM, 43.1 µM, 9.4 µM and 24.0 µM, respectively. Linoleic acid, osthenol, falcarindiol and 11 (S), 16(R)-dihydroxyoctadeca-9Z,17-diene-12,14-diyn-1-yl acetate exhibited inhibitory activity on COX-1 with IC50 values of 13.3 µM, 64.3µM, 66.0 µM and 73.3 µM.