2D-6 In Vivo Characterisation of Microbubbles using High Frequency Ultrasound

The goal of this project was to determine the utility of microbubble contrast agents in relation to mouse imaging with high frequency ultrasound. The ability to perform quantitative haemodynamic measurements using high frequency ultrasound and microbubbles is dependent on the behaviour of the contrast agent in vivo. The objective of this study was to investigate the acoustic response and kinetic behaviour of the clinical contrast agent Definitytrade in mice exposed to 30 MHz ultrasound. In vitro experiments showed that the agent behaved predominantly as linear scatterers for pressures50 muL/kg. It is believed that the utility of microbubble contrast agents for quantitative flow imaging in the mouse using high frequency ultrasound is in 5 to 50 muL/kg dose range. To summarize, the characteristics of Definitytrade injected in a mouse and exposed to 30 MHz ultrasound have been investigated