Trace Elements for Growth and Bulbiformin Production by Bacillus subtilis

S ummary : The elements Mn and Fe, both alone and together, had a marked effect on growth and bulbiformin production by Bacillus subtilis. Copper, with Fe and/or Mn had a deleterious effect on antibiotic production, whereas Cu+Zn as well as Mo+B slightly increased the antibiotic produced over that obtained with Fe+Mn. The optimum requirement of Fe was 20 p/m for both growth and antibiotic activity. The requirement of Mn for maximum growth was higher (2 p/m) than that for antibiotic activity (1 p/m).