The expression of CsLFY and bar genes at transcription and translation levels in transgenic tobacco plants

Transgenic tobacco plants containing CsLFY and bar under 35S CaMV promoter control in T 1 generation were analyzed. Also, the presence and expression of these transgenes at molecular level were studied. The presence of the transgenes in the genome was shown by PCR and expression of these genes at the level of transcription and translation processes was investigated. Expression of these differential transgenes at RNA level was shown in all lines. Electrophoretic patterns revealed the similarity of protein spectra of transgenic lines and the tobacco control. Proteins with molecular mass 44 kDa and 23 kDa were detected in different plant organs (mainly in leaves, leafstalk, stalk and less in roots) which theoretically correspond to expression products of CsLFY and bar genes, showing their constitutive activity.