AIE Fluorescent Nanozyme-Enabled Ratiometric Sensing Platform for Selective Detection of Ascorbic Acid and Alkaline Phosphatase

-phenylenediamine (OPD), ZIF-8/Ru@TPE catalyzed OPD oxidation to form the fluorescent product 2,3-diaminophenazine (DAP), yielding a distinct emission peak at 570 nm while quenching the TPE fluorescence at 445 nm. Notably, AA triggered a self-amplified ratiometric response by simultaneously inhibiting OPD oxidation and generating an additional fluorophore through the reaction of dehydroascorbic acid with OPD, achieving high selectivity over other reductants. Exploiting ALP-catalyzed AA production, this platform was successfully extended to ALP activity determination in serum. This study presents a versatile nanozyme-based ratiometric sensing strategy for selective detection of AA and ALP, offering great potential for accurate bioanalysis in complex samples.