Context-Dependent Effects of Platelet-Derived Growth Factor on Osteogenesis in 2D and 3D Fibrin Models

This study demonstrates the context- and cell population-dependent ability of PDGF-bb to induce osteogenic mineralization in 2 distinct in vitro models. The findings suggest that PDGF-bb's mitogenic activity promotes proliferation and matrix deposition in 2D environments, but its effects may be limited in 3D fibrin constructs where we postulate that spatial constraints restrict proliferation. The evaluation of PDGF-bb, and other newly developed adjuvants, in both 2D and 3D in vitro assays is required to determine context-dependent osteogenesis. This understanding provides a basis for future experiments to assist in determining the ideal patients for utilization of PDGF-bb and determine if there are instances when other adjuvants may be considered.