Evaluations of Trichloroacetic Acid-acetone Method and Phenol Method for Protein Extraction from Cotton Ovule

Trichloroacetic acid-acetone precipitation method (TCA method) and phenol isolation method (phenol method) were evaluated by measuring the yield ratio and purity of protein and analysis of the gel electrophoresis map on ovule of DPL971 and DPL972 in Gossypium arboreum during primary cell wall synthesis period. The results were as follows: firstly, the massive soluble proteins could be obtained by the two methods, but the yield ratio, quality and protein types by phenol method were better than those by TCA method; secondly, the types of extracted protein were different by two methods, the phenol method had better performance in acidic region and in high/low molecular weight region; finally, the phenol method could efficiently get rid of the interfering substance and achieve the excellent gels, therefore, the phenol method was suitable for protein extraction from the cotton ovule. As a result, the useful information can be provided for the proteomics research of cotton fiber during the development period and have certain significance for guiding the protein extraction of many other non-model plants containing various interfering elements.