张力素
PTEN公司
癌症研究
癌变
小RNA
细胞生长
下调和上调
骨肉瘤
磷酸酶
MAPK/ERK通路
信号转导
蛋白激酶B
PI3K/AKT/mTOR通路
生物
细胞生物学
磷酸化
车站3
细胞凋亡
癌症
遗传学
基因
作者
Weiguo Wang,Zhengguang Wang,Shi-Jie Chen,Xiaofang Zang,Jinglei Miao
摘要
So far, microRNA has attracted plenty of interest due to its role in tumorigenesis. Reportedly, miR-181b may be involved in the tumorigenesis of osteosarcoma (OS). In the current study, we attempted to investigate the detailed function and mechanism of miR-181b in OS carcinogenesis. Herein, miR-181a, miR-181b, miR-181c, and miR-181d expressions in OS tissues were higher than that in nontumor tissue samples as examined real-time polymerase chain reaction. Via direct targeting, miR-181b negatively regulated the expression of phosphatase and tensin homolog (PTEN), a well-known tumor suppressor. Furthermore, a small interfering RNA strategy was used to find that interleukin (IL)-1B and nuclear factor-κB (NF-κB) regulate miR-181b and PTEN expression. Consequently, the repression of PTEN by miR-181b promotes OS cell proliferation. In summary, our data support a critical role for NF-κB-dependent upregulation of miR-181b, which further inhibited PTEN expression and promoted the cell proliferation of OS cell lines. The above findings represent a new pathway for the repression of PTEN and the promotion of cell proliferation upon IL-1β induction.
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