MP074PROTECTIVE EFFECT OF NITRIC OXIDE RELEASING NANOFIBER ON RENAL ISCHEMIA-REPERFUSION INJURY IN RATS

INTRODUCTION AND AIMS: Renal ischemia-reperfusion injury (IRI) is very important in various clinical setting including renal transplantation. Nitric oxide (NO) is well known for having various protective effects on cells during IRI. However, there was no way to properly deliver NO to the target organ. Recently, S-Nitrosothiol-modified silica/polymer hybrid nanofibers have been introduced as a NO storage and delivery nanoscaffold. The aim of this study was to investigate the effect of NO releasing nanofiber on renal IRI in rat. METHODS: Fifteen male Sprague-Dawley rats were divided into three groups: 1) sham group (n=5); 2) control group, renal IRI without any treatment (n=4); and 3) NO group, renal IRI with wrapping the kidney using NO rapid releasing-polymer nanofiber matrix (n=6). Right nephrectomy was done one week before renal IRI. NO releasing sheet was applied by wrapping left kidney one hour before clamp of renal artery. Renal ischemia was sustained during 55 minutes, followed by reperfusion. NO sheet was removed after 24 hours. The rats were sacrificed 48 hours after surgery. RESULTS: To determine the effect of NO on kidney function, we analyzed serum creatinine level. There was a significant difference of creatinine level between three groups (0.48 ± 0.08, 4.67 ± 0.33, and 2.60 ± 1.0 respectively, p=0.002). We also analyzed markers for inflammation, oxidative stress and apoptosis including ICAM-1, VCAM-1, TNF-α, IL-1b, 6 and 9, NFkb, cleaved caspase 3, Cox-2, iNOS, ED1, and BAX/Bcl-2 ratio in kidney tissue. Most of these markers increased significantly more in the control group as compared with the NO group (p<0.05). In histopathological analysis of kidney tissue, NO group had significantly lower tubulointerstitial injury score than the control group (p<0.05). CONCLUSIONS: In this study, we demonstrate the protective effect of exogenous NO on renal IRI. This finding might be correlated with inflammation, oxidative stress and apoptosis. Furthermore, this new NO delivery system might be considered as a novel method for ameliorating IRI in renal transplantation.