1α,25( OH)_2D_3 inhibits proliferation of gastric cancer cell line by arresting cell cycle

Objective To observe the effect of 1α,25( OH)2D3on gastric carcinoma cell proliferation and cell cycle progression and to investigate its possible mechanism. Methods Two gastric cancer cell lines BGC-823 and SGC-7901 were treated with 1α,25( OH)2D3in a dose dependent manner. Cell proliferation was determined by methyl thiazolyl tetrazolium MTT assay; the cell cycle changes were detected by using flow cytometry; the expression of genes of P21,cyclin D1,cyclin E1 and CDK6 related to the cell cycle was analyzed by RT-PCR. Results1α,25( OH)2D3significantly inhibited two gastric cancer cell lines proliferation in a dose dependent fashion,gastric cancer cells treated with 1α,25( OH)2D3caused significantly cell cycle arrest at G1phase; RT-qPCR showed that 1α,25( OH)2D3significantly increased P21 mRNA,and attenuated cyclin D1,cyclin E1,CDK6 mRNA expression in BGC-823,SGC-7901 cells( P 0. 01). Conclusions 1α,25( OH)2D3inhibites BGC-823,SGC-7901cell proliferation and induces cell cycle arresting in G1phase. The mechanism for the anti-proliferative and cell cycle arrest is probably related to overexpression of P21,down-regulated expression of cyclin D1,cyclin E1,CDK6.