The impact of humic acid substances in different regions on the flocculation of metal ions is studied in this paper.The structure and complex nature of humic acid substances are analyzed by IR spectra.The results show that humic acid substances have a strong complexing adsorption capacity on metal ions,namely,a gram of humic acid products can adsorb a few hundred milligrams of metal ions.Humic acid substances in different regions have different ability on metal ion complexation.The metal flocculation ability of sodium humate products of Yunnan Eshan is better than other humic acid substances.The complex adsportion reaction occurs mainly in response to the active site carboxyl and phenolic hydroxyl group of the substances.The study on the anti-flocculation ability of humic acid substances with metal ions has important theoretical significance and application value on how to avoid the generation of insoluble precipitate humic acid substances in agriculture and medicine and other fields of application process.